Overview

  • Product name
    Rat Albumin ELISA Kit
    See all Albumin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.5%
  • Sample type
    Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.7 µg/ml
  • Range
    0.78 µg/ml - 50 µg/ml
  • Recovery

    98 %

  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Albumin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Albumin in plasma and serum.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Rat albumin measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108790.

Protocols

References

This product has been referenced in:
  • Zhang Y  et al. Change in the Lipid Transport Capacity of the Liver and Blood during Reproduction in Rats. Front Physiol 8:517 (2017). Read more (PubMed: 28798692) »
  • Arellano-Buendía AS  et al. Urinary excretion of neutrophil gelatinase-associated lipocalin in diabetic rats. Oxid Med Cell Longev 2014:961326 (2014). Read more (PubMed: 25243053) »

See all 2 Publications for this product

Customer reviews and Q&As



I can confirm that 25ul sample per well is used:

The sample is plasma collected using one-tenth volume of 0.1 M sodium citrate as an anticoagulant. Centrifuge samples at 2000 x g for 10 minutes and assay. Dilute samples 1:8000 into ...

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Thank you for contacting us.

Unfortunately we only sell the kits as complete units. These come in from the lab pre-assembled and there is no way for us to split apart kits to sell separately.

I apologize that this information coul...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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