Overview

  • Product name
    Rat BNP 45 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.03 ng/ml
  • Range
    0.031 ng/ml - 1 ng/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, tissue extract, and cell culture supernatants.

    A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.

  • Notes

    There is significant cross-reactivity between BNP32 and BNP45.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Rat BNP 45 Antibody 1 x 120µl
    BNP 45 Microplate (12 x 8 well strips) 1 unit
    BNP 45 Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity
    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities
    Belongs to the natriuretic peptide family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • BNP-45
    • Gamma-brain natriuretic peptide
    • nppb
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Standard curve : mean of duplicates (+/-SD) with background readings subtracted

  • Rat BNP45 measured in biological fluids showing quantity (pg) per mL of tested sample

Protocols

References

This product has been referenced in:
  • Youcef G  et al. Simultaneous characterization of metabolic, cardiac, vascular and renal phenotypes of lean and obese SHHF rats. PLoS One 9:e96452 (2014). Read more (PubMed: 24831821) »
  • Wang YB  et al. Rehmannia glutinosa extract activates endothelial progenitor cells in a rat model of myocardial infarction through a SDF-1 a/CXCR4 cascade. PLoS One 8:e54303 (2013). ELISA ; Rat . Read more (PubMed: 23349848) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

Currently, this is the only BNP 45 ELISA kit (ab108816) which recognizes rat BNP 45. We sell primary antibodies which detect human BNP:

ab20984,

ab19645,

ab19646,

ab47699, Read More

Rat BNP 45 ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews
Rat BNP 45 ELISA Kit
The ELISA Kit is easy to use and works well.
Rat plasma was used with 10 times dilution.(For reference)
Antibody incubation time is a bit long.
Username

Abcam user community

Verified customer

Submitted Jul 10 2017

Our samples showed around 40% cross-reactivity for rabbit protein.

Merci de nous avoir contactés.

Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

1) Les deux kits peuvent en effet êtr...

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Vielen Dank für Ihren Anruf.

Wenn das Futter keinen Einfluss auf die Produktion dieser Peptide hat (wie durch zB salzreiche Nahrung), sehe ich keinen Grund, warum die Ratten fasten sollten.

Ich hoffe, dies hilft Ihnen weiter....

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Thank you for contacting us.

The best way to test the viability is using the kit. The kits should be fine at -20C so please use the kit without any hesitation.

I hope this information is helpful to you. Please do not hesitate to conta...

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Thank you for your reply and the additional information.

We have the following PO on file: xxx.The order was from xxx and the lot number matches what you had sent, thus I think this is the correct order.


I have now arranged t...

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Thank you for contacting us.

I am very sorry to hear about the problems your customer had to face because of the second kit. I sincerely apologize about that! Yes, I'd be happy to arrange for a free of charge replacement.


Cou...

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Gracias por contactarnos y alertarnos de este fallo.



Lamento mucho que el vial de estándar del nuevo lote enviado no indicara ningún dato acerca de la cantidad contenida. Sin duda se trata de un error que estamos trata...

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Thank you for your enquiry and your interest.

As the Protocol booklet indicates we would suggest adding 50 μl of Chromogen Substrate per well and incubate for about 15 minutes or till the optimal blue colour density develops. The optimal t...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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