Overview

  • Product name
    Rat Ceruloplasmin ELISA Kit (CERP)
    See all Ceruloplasmin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7%
  • Sample type
    Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.7 µg/ml
  • Range
    0.78 µg/ml - 50 µg/ml
  • Recovery

    96 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Ceruloplasmin (CERP) Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in plasma and serum.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Ceruloplasmin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Ceruloplasmin captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108820.

Protocols

References

ab108820 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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