Overview

  • Product nameRat Ceruloplasmin ELISA Kit (CERP)
    See all Ceruloplasmin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.7 µg/ml
  • Range
    0.78 µg/ml - 50 µg/ml
  • Recovery

    96 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Ceruloplasmin (CERP) Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin in plasma and serum.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Ceruloplasmin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Ceruloplasmin captured in plate.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCeruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similaritiesBelongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CERU_HUMAN
    • Ceruloplasmin
    • CP
    • CP 2
    • CP2
    • Ferroxidase
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Rat Ceruloplasmin ELISA Kit (CERP) images

  • Representative Standard Curve using ab108820.

Protocols

References for Rat Ceruloplasmin ELISA Kit (CERP) (ab108820)

ab108820 has not yet been referenced specifically in any publications.

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