Overview

  • Product name

    Rat CINC-1 ELISA Kit (CXCL1)

  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Intra-assay < 10%
    Inter-assay
    Sample n Mean SD CV%
    Inter-assay < 12%
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.05 ng/ml
  • Range
    0.047 ng/ml - 15 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112.5 99% - 122%
    Plasma 107.9 97% - 125%

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s CINC-1 (CXCL-1) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-1 in serum, plasma and cell culture supernatants. (Collect plasma using EDTA and heparin as an anticoagulant). Citrate is not recommended as anticoagulants for use in this assay. Rat CINC-1 concentration in normal plasma is very low and it may not be detected in this assay.


    This assay employs an antibody specific for rat CINC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CINC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat CINC-1 in normal serum with ab155462, only in serum spiked with Rat CINC-1.

  • Notes

    Sensitivity of the assay is dependant on the Assay Diluent used. Please refer to Protocol Booklet for more details.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155462 - Assay Diluent D.

  • Representative standard curve using ab155462 -  Assay Diluent B.

Protocols

References

ab155462 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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