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|Sample type||Average %||Range|
|Plasma||91.87||72% - 111%|
|Cell culture media||83.48||75% - 89%|
Abcam's CINC-3 (CXCL2) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat CINC-3 (CXCL2) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CINC-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CINC-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CINC-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CINC-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|600X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-rat CINC-3||2 vials|
|CINC-3 Microplate (12 x 8 wells)||1 unit|
|Recombinant Rat CINC-3 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155463 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|