Overview

  • Product nameRat CNTF ELISA Kit
    See all CNTF kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    8.23 pg/ml - 2000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.3 89% - 108%
    Serum 105.6 93% - 112%
    Plasma 104.2 92% - 110%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s CNTF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat CNTF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCiliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.
  • Cellular localizationCytoplasmic
  • Alternative names
    • AI429687
    • Ciliary Neurotrophic Factor
    • HCNTF
    • MGC41235
    • OTTHUMP00000174731
    • OTTMUSP00000029848
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat CNTF ELISA Kit images

  • Representative standard curve using ab100758

  • Representative standard curve using ab100758

Protocols

References for Rat CNTF ELISA Kit (ab100758)

ab100758 has not yet been referenced specifically in any publications.

Product Wall

Epitope mapping has not been performed so the exact binding sequence that the antibody pair recognizes is not known. All that is known is that the antibodies are binding somewhere on the full length protein (http://www.uniprot.org/uniprot/P20294).

Abreview for CNTF Rat ELISA Kit

Average Average 3/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (blood)
Specification: blood
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: rat serum
purified CNTF
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Wanted to use the kit to measure CNTF in mouse serum, but it did not work for the mouse samples. Mouse serum was used in multiple amounts, but the plate wells containing the mouse samples were no different than background. The kit worked well with serial dilutions of my positive control (rat serum), though. The kit is otherwise easy to use.
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Verified customer

Submitted Dec 21 2011

Thank you for your inquiry.  Unfortunately, we have not tested mouse samples with the ab99994 kit or ab100758 kit. The lab didn't think that the human kit ab99994 would detect mouse samples since it didn't work in rat (the mouse and rat proteins ar...

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Thank you for your email.  I have addressed your question below. ab100758 : No cross reactivity data is available testing mouse samples with this rat kit therefore the only way to know would be to test and see.  However, both the capture and detection ...

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I am very pleased to hear you would like to accept our offer and test ab100758 in mouse. This code will give you 1 free kit OR 1 free antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"