• Product nameRat CXCL5 ELISA Kit
    See all CXCL5 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 91.25 82% - 102%
    Tissue Extracts 93.47 84% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s CXCL5 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL5 cell lysate and tissue lysate.

    This assay employs an antibody specific for Rat CXCL5 coated on a 96- well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceCXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
  • Alternative names
    • AMCF II
    • AMCFII
    • C X C motif chemokine 5
    • Chemokine (C X C motif) ligand 5
    • CXCL 5
    • ENA 78
    • ENA78
    • Epithelial derived neutrophil activating protein 78
    • GCP 2
    • LIX
    • Neutrophil activating peptide ENA 78
    • Neutrophil activating protein 78
    • SCYB5
    • Scyb6
    • Small inducible cytokine B5
    • Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78)
    • Small inducible cytokine subfamily B, member 15
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat CXCL5 ELISA Kit images

  • Representative standard curve using ab100775


References for Rat CXCL5 ELISA Kit (ab100775)

ab100775 has not yet been referenced specifically in any publications.

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