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|Sample type||Average %||Range|
|Urine||107||101% - 113%|
|Serum||116||111% - 119%|
|Citrate Plasma||115||113% - 117%|
|Fetal Bovine Serum||96||94% - 96%|
|1X Cell Extraction Buffer PTR||86||85% - 88%|
|serum free media||96||95% - 98%|
Abcam’s E-Cadherin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of E-Cadherin protein in rat serum, plasma, urine and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
E-Cadherin is a member of the Cadherin family of calcium-dependent cell adhesion proteins. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.
|Components||1 x 96 tests|
|10X Rat E-Cadherin Capture Antibody||1 x 600µl|
|10X Rat E-Cadherin Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 5BC||1 x 6ml|
|Plate Seals||1 unit|
|Rat E-Cadherin Lyophilized Recombinant Protein||2 vials|
|Sample Diluent NS||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab202413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 983.9 ng/mL in serum, 620.7 ng/mL in plasma (Citrate), and 1,771 ng/mL in urine.
ab202413 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"