Overview

  • Product name
    Rat E-Cadherin ELISA Kit
    See all E Cadherin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.6%
  • Sample type
    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    17 pg/ml
  • Range
    156.25 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 107 101% - 113%
    Serum 116 111% - 119%
    Citrate Plasma 115 113% - 117%
    Fetal Bovine Serum 96 94% - 96%
    1X Cell Extraction Buffer PTR 86 85% - 88%
    serum free media 96 95% - 98%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Rabbit, Goat, Guinea pig, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s E-Cadherin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of E-Cadherin protein in rat serum, plasma, urine and cell culture supernatant.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    E-Cadherin is a member of the Cadherin family of calcium-dependent cell adhesion proteins. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Rat E-Cadherin Capture Antibody 1 x 600µl
    10X Rat E-Cadherin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BC 1 x 6ml
    Plate Seals 1 unit
    Rat E-Cadherin Lyophilized Recombinant Protein 2 vials
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • Research areas
  • Relevance
    One of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.
  • Alternative names
    • Arc 1
    • Cadherin 1
    • cadherin 1 type 1 E-cadherin
    • Cadherin1
    • CAM 120/80
    • CD 234
    • CD324
    • CD324 antigen
    • CDH1
    • CDHE
    • ECAD
    • Epithelial cadherin
    • epithelial calcium dependant adhesion protein
    • LCAM
    • Liver cell adhesion molecule
    • UVO
    • Uvomorulin
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab202413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 983.9 ng/mL in serum, 620.7 ng/mL in plasma (Citrate), and 1,771 ng/mL in urine.

Protocols

References

ab202413 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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