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|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam's Ferritin (FTL) Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of ferritin in rat biological samples.
In this assay the ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|100X HRP-conjugated anti-rat Ferritin antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Rat Ferritin Calibrator (Lyophilized)||1 vial|
|Rat Ferritin ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab157732 has not yet been referenced specifically in any publications.