|Sample type||Average %||Range|
|Serum||93||% - %|
|Tissue Culture Media||92||% - %|
|EDTA Plasma||95||% - %|
Abcam’s Gastrin 1 Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Gastrin 1 in tissue.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Gastrin I antigen and a polyclonal rabbit antibody specific to Gastrin I. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the coloration is inversely proportional to the amount of Gastrin I captured in the plate.
Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion. Rat Gastrin I (G17) Pyr-Arg-Pro-Pro-Met-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2. Gastrin is synthesized as a 104 residue pre-pro-peptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34 and G17. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. G34 is the major circulating Gastrin in fasting serum, but with G17, increases two to threefold after feeding until both are present in approximately equal amounts. Normal serum levels in rats have been reported over a range of 40-169 pg/mL with possible variations between animal sources.
|Compound||Cross Reactivity (%)|
|Cholecystokinin 26-33 (CCK-8)||100|
|Human Gastrin I||30|
|Mouse Gastrin I||20.5|
|Gastrin Tetrapeptide (CCK-4)||0.75|
Gastrin Inhibitory Polypeptide (GIP)
Gastrin Releasing Peptide (GRP)
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 30ml|
|Assay Buffer||1 x 30ml|
|Gastrin I Antibody||1 x 6ml|
|Gastrin I Alkaline Phosphatase Conjugate||1 x 6ml|
|Goat anti-rabbit IgG Microplate (12 x 8 wells)||1 unit|
|Plate Sealer||1 unit|
|pNpp Substrate||1 x 23ml|
|Rat Gastrin I Standard||1 x 0.25ml|
|Stop Solution||1 x 6ml|
Our Abpromise guarantee covers the use of ab133049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|