Overview

  • Product name
    Rat Gastrin 1 ELISA Kit
    See all Gastrin I kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low 24 79.9pg/ml 8.3%
    Medium 24 260pg/ml 8.8%
    High 24 937pg/ml 8.5%
    Inter-assay
    Sample n Mean SD CV%
    Low 12 86.5pg/ml 14.7%
    Medium 12 233pg/ml 12.6%
    High 12 904pg/ml 7%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue Culture Media
  • Assay type
    Competitive
  • Sensitivity
    78.1 pg/ml
  • Range
    78.1 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 % - %
    Tissue Culture Media 92 % - %
    EDTA Plasma 95 % - %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Gastrin 1 Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Gastrin 1 in tissue.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Gastrin I antigen and a polyclonal rabbit antibody specific to Gastrin I. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the coloration is inversely proportional to the amount of Gastrin I captured in the plate.

  • Notes

    Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G Cells of the antrum mucosa in response to a number of stimuli associated with digestion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G Cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels will also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions. Gastrin is the most potent stimulator of gastric acid secretion. Rat Gastrin I (G17) Pyr-Arg-Pro-Pro-Met-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2. Gastrin is synthesized as a 104 residue pre-pro-peptide on the rough endoplasmic reticulum, then post-translationally modified by cleavage and alpha-amidation to result in the active forms G34 and G17. Other forms also exist, but are not considered biologically significant. There are two types of G17 and G34, type II is sulfated at the tyrosine residue, while type I is not. Both G34 and G17 circulate and contribute to the stimulation of gastric acid secretion, but have different clearance rates. G34 is the major circulating Gastrin in fasting serum, but with G17, increases two to threefold after feeding until both are present in approximately equal amounts. Normal serum levels in rats have been reported over a range of 40-169 pg/mL with possible variations between animal sources.

     

    CompoundCross Reactivity (%)
    Rat Gastrin100
    Cholecystokinin 26-33 (CCK-8)100
    Human Gastrin I30
    Mouse Gastrin I20.5
    Minigastrin (G13-I)11
    Gastrin Tetrapeptide (CCK-4)0.75
    Glucagon0.5

    Gastrin Inhibitory Polypeptide (GIP)

    <0.2
    Bombesin<0.2
    Somatostatin-14<0.2

    Gastrin Releasing Peptide (GRP)

    <0.2
  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133049.

Protocols

References

This product has been referenced in:
  • Na K & Kim HS Adrenalectomy abolishes hypergravity-induced gastric acid hyposecretion. Oncotarget 8:30700-30705 (2017). Functional Studies ; Rat . Read more (PubMed: 28430608) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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