Rat heart tissue lysate - mitochondrial extract (ab110347)

Overview

  • Product name
    Rat heart tissue lysate - mitochondrial extract
    See all Rat Heart lysates
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • Tested applications
    Suitable for: SDS-PAGE, WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • Lysate notes
    2 mg of rat heart mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE. Immunocapture, however could also be used for electrophoresis and Western blotting, and Enzyme Isolations. 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

Applications

Our Abpromise guarantee covers the use of ab110347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References

ab110347 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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