|Sample type||Average %||Range|
|Cell culture supernatant||99.29||89% - 111%|
|Plasma||92.63||78% - 104%|
Abcam’s IFN gamma Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IFN gamma in plasma and cell culture supernatants. (Rat IFN gamma concentration in normal plasma is pretty low, it may not be detected in this assay).
This assay employs an antibody specific for IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IFN gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Rat IFN-gamma in normal serum with ab113349, only in serum spiked with Rat IFN-gamma.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-rat IFN Gamma (lyophilized)||2 vials|
|IFN gamma Microplate (12 x 8 wells)||1 x 96 units|
|Recombinant rat IFN gamma Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113349 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113349 has not yet been referenced specifically in any publications.