Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

Overview

  • Product nameRat IgG1, kappa monoclonal [RTK2071] - Isotype control
    See all Rat isotype controls
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cyt, WB, IPmore details
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes


    The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Isotype control notesThe ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • ClonalityMonoclonal
  • Clone numberRTK2071
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 2µg for 106 cells. ab18412 is a negative isotype control and can be used for surface or intracellular staining
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Rat IgG1, kappa monoclonal [RTK2071] - Isotype control images

  • Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

ab18412 has not yet been referenced specifically in any publications.

Product Wall


Our Rat IgG1, kappa monoclonal Isotype control is a suitable isotype control for ab13789 and has been tested and guaranteed to work in IHC-frozen. A link for this isotype control is given below:
ab18412 (http://www.abcam.com/Rat-IgG1-kappa-mon...

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Please find attached to this email the MSDS you requested for the following antibodies:
ab3104
ab18412
ab45139
ab68672

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"