Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
Related conjugates and formulations
Overview
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Product name
Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control
See all Rat isotype controls -
Conjugation notes
Please note that this antibody is unconjugated. -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details -
Immunogen
Trinitrophenol + KLH
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General notes
ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS
0.2 µm filter sterilized -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The immunoglobulin was purified by affinity chromatography. -
Isotype control notes
ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. -
Clonality
Monoclonal -
Clone number
RTK2758 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
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Alternative names
- Rat Isotype Control
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use 2µg for 106 cells.
ab18450 is a negative isotype control. |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use 2µg for 106 cells. ab18450 is a negative isotype control. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Images
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Overlay histogram showing HeLa cells stained with ab53358 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53358, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab18450 has been referenced in 12 publications.
- Santini MP et al. Tissue-Resident PDGFRa+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner. Cell Rep 30:555-570.e7 (2020). PubMed: 31940496
- Saraswati S et al. Small proline-rich repeat 3 is a novel coordinator of PDGFRß and integrin ß1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts. FASEB J 34:7885-7904 (2020). PubMed: 32297675
- Chang HR et al. Role of LpL (Lipoprotein Lipase) in Macrophage Polarization In Vitro and In Vivo. Arterioscler Thromb Vasc Biol 39:1967-1985 (2019). PubMed: 31434492
- Hordeaux J et al. The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier. Mol Ther 27:912-921 (2019). PubMed: 30819613
- Mantani PT et al. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol 20:47 (2019). PubMed: 31823769