Rat IgG2a, kappa monoclonal [RTK2758] (FITC) - Isotype control (ab18446)

Q&A (3)Specific References (4)
Customer reviews and FAQs

Overview

  • Product name
    Rat IgG2a, kappa monoclonal [RTK2758] (FITC) - Isotype control
    See all Rat isotype controls
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab18446 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues
  • Tested applications
    Suitable for: Flow Cytmore details
  • General notes


    ab18446 RTK2758 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Purity
    Whole antiserum
  • Purification notes
    The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
  • Isotype control notes
    ab18446 RTK2758 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality
    Monoclonal
  • Clone number
    RTK2758
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

References for Rat IgG2a, kappa monoclonal [RTK2758] (FITC) - Isotype control (ab18446)

This product has been referenced in:
  • Xia X  et al. Inhibitor of DNA binding 1 regulates cell cycle progression of endothelial progenitor cells through induction of Wnt2 expression. Mol Med Rep N/A:N/A (2016). Read more (PubMed: 27432753) »
  • Kjær I  et al. Cetuximab Resistance in Squamous Carcinomas of the Upper Aerodigestive Tract Is Driven by Receptor Tyrosine Kinase Plasticity: Potential for mAb Mixtures. Mol Cancer Ther 15:1614-26 (2016). Read more (PubMed: 27196767) »

See all 4 Publications for this product

Publishing research using ab18446? Please let us know so that we can cite the reference in this datasheet.

Product Wall

Submit a question for ab18446

3 Q&A

10
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I have a question regarding the following product that I bought:
Rat IgG2a, kappa monoclonal [RTK2758] (FITC) - Isotype control
Cat. Nr.: ab18446
At with concentration is sailed this antibody?
This is important to understand some strang...

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Thank you for contacting us.
As shown on the product datasheet, www.abcam.com/ab18446, this product is a whole antiserum.
Unpurified antibodies will not have a concentration stated on the datasheet. For whole antiserum (such as ab18446), cult...

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Product code: 11400
Inquiry: Hi, I am looking for an antibody against EGFR (ErbB1) to use on human and mouse cell lines for FACS with a FITC label and I have found ICR10. I was wondering if you could tell me which antibody to buy to use an an Isot...

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Thank you for contacting us.

The only isotype control we can offer is ab18446; http://www.abcam.com/Rat-IgG2a-kappa-monoclonal-RTK2758-FITC-Isotype-control-ab18446.html

Please note that the concentration of isotype is not known be...

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Purchased wrong antibody ab18703 and ab18450. How could we return the antibodies? We are interested in getting ab18446 and rat monoclonal anti IgG2c antibody which we could not find in catalogue.

Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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