Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free) (ab18450)

Overview

  • Product name
    Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free)
    See all Rat isotype controls
  • Conjugation notes
    Please note that this antibody is unconjugated.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Frmore details
  • Immunogen

    Trinitrophenol + KLH

  • General notes


    ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
    0.2 µm filter sterilized. Endotoxin level is <0.01 ng/µg protein
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab18450 is LEAF™ Purified. This a low endotoxin – sodium azide free formulation. ab18450 was 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein. LEAF™ Purified formulation is typically used for bioassays, capture antibody for ELISPOT application, and in vivo studies such as Blocking/Neutralization.
  • Isotype control notes
    ab18450 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality
    Monoclonal
  • Clone number
    RTK2758
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab18450is a negative isotype control



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References for Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free) (ab18450)

This product has been referenced in:
  • Daschkey S  et al. MicroRNAs distinguish cytogenetic subgroups in pediatric AML and contribute to complex regulatory networks in AML-relevant pathways. PLoS One 8:e56334 (2013). IP . Read more (PubMed: 23418555) »
  • Hausmann M  et al. BCL-2 Modifying Factor (BMF) Is a Central Regulator of Anoikis in Human Intestinal Epithelial Cells. J Biol Chem 286:26533-40 (2011). Read more (PubMed: 21673109) »

See all 3 Publications for this product

Product Wall

Expiration date: 25-04-2012
I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
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The subisotype ofab86488 is IgG2a. Thus, the recommended isotype control to be used in flow cytometry would be ab18450.

I hope this information is helpful to you. Please do not hesitate to contact us i...

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Flow cytometry has not yet been tested with this antibody. Therefore, we do not have data to show if and how well it would work in this application. But for UNTESTED species and/or applications, we have establish...

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Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice p...

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Thank you for your enquiry. To our knowledge this antibody has yet to be tested in-vivo. If you decide to purchase this antibody for testing in applications for which we don't have any information, please let us know how you get on. In return we will a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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