Rat IgG2b, kappa monoclonal [RTK4530] - BSA/Azide free (ab18536)

Overview

  • Product name
    Rat IgG2b, kappa monoclonal [RTK4530] - BSA/Azide free
    See all Rat isotype controls
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC, IP, Functional Studiesmore details
  • Immunogen

    Trinitrophenol + KLH

  • General notes
    Isotype control has been screened on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Solution contains no preservative; handle under aseptic conditions.
  • Clonality
    Monoclonal
  • Clone number
    RTK4530
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Rat Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test

References

ab18536 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

Unfortunately ab18536 and ab18541 have not been tested in ChIP. It doesn’t mean they won’t work in this application, but we don’t have data to support it.

If you are interested in parti...

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Thank you for your inquiry.

I am sorry to confirm that ab37361 is not aa IgG2b antibody but a mixture of rat IgGs of all isotypes.

I can suggest to following antibody for your customer's experiments:

ab18536 (Rat IgG2b, ka...

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This antibody is 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL (Limulus amebocyte lysate) test. If you have any other questions, please do not hesitate to ask.

Thank you for your enquiry. I have been in touch with the lab and I have determined that this antibody was generated by immunisation of naïve rat with TNP+KLH and fused with FO mouse myeloma cell line. The isotype of this antibody is IgG2b, k (test...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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