Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
Our Abpromise guarantee covers the use of ab35774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution.|
ab35774 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"