Other Immunogen Type corresponding to Rat. Trinitrophenol + KLH
Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
Our Abpromise guarantee covers the use of ab35768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. ab35768 is a negative isotype control|
|WB||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution.|