Overview

  • Product name
    Rat IL-1 alpha ELISA Kit (Interleukin-1 alpha)
    See all IL1 alpha kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    11 pg/ml - 8000 pg/ml
  • Recovery

    81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 87.48 76% - 95%
    Serum 79.95 69% - 88%
    Plasma 75.73 68% - 84%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-1 alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 alpha in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab113350

Protocols

References

This product has been referenced in:
  • Zhang X  et al. Risk of radiation-induced pneumonitis after helical and static-port tomotherapy in lung cancer patients and experimental rats. Radiat Oncol 10:195 (2015). Read more (PubMed: 26382926) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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