Overview

  • Product name
    Rat IL-1 beta ELISA Kit (Interleukin-1 beta)
    See all IL1 beta kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    68.59 pg/ml - 50000 pg/ml
  • Recovery

    99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 92.89 81% - 109%
    Serum 107.2 95% - 115%
    Plasma 97.55 89% - 108%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-1 beta (Interleukin-1 beta) Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat IL-1 beta 2 vials
    IL-1 beta Microplate (12 x 8 wells) 1 unit
    Recombinant rat IL-1 beta Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Domain
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100767

  • Representative standard curve using ab100767

Protocols

References

This product has been referenced in:
  • Ho D  et al. Maternal-placental-fetal biodistribution of multimodal polymeric nanoparticles in a pregnant rat model in mid and late gestation. Sci Rep 7:2866 (2017). Read more (PubMed: 28588270) »
  • Jiang YH  et al. Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation. PLoS One 10:e0133971 (2015). Read more (PubMed: 26230083) »

See all 3 Publications for this product

Customer reviews and Q&As

Perdona por la espera.
Es muy posible que el motivo de la baja absorbancia observada en las placas se deba a la perdida de actividad de alguno de los componentes del kit, debido a su degradación por haberse mantenido durante tantos meses a 4C. Read More

Gracias por mantenerte a la espera.
Después de discutir el caso con mis compañeros, hemos comentado lo siguiente:
- Ambos kits se pidieron en Junio, si no se han utilizado hasta ahora es posible que haya habido degradación de los reactivos de...

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Thank you for contacting us. The host of the detection antibody is goat, and the host of the capture antibody is Armenian hamster. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or info...

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Thank you for your inquiry. I am happy to confirm that we guarantee our products for six month if stored correctly. ab100767 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein)...

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Thank you for your enquiry. I can confirm that ab100767 IL1 beta Rat ELISA Kit would be the suitable kit to use with plasma samples. ab100768 IL1 beta Rat ELISA Kit is not designed for use with plasma samples. I hope this will be helpful to yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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