Overview

  • Product name

    Rat IL-1 beta ELISA Kit (Interleukin-1 beta)


    See all IL1 beta kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    68.59 pg/ml - 50000 pg/ml
  • Recovery

    116 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 96.22 88% - 105%
    Tissue Extracts 136.5 126% - 145%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-1 beta (Interleukin-1 beta) Rat ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta in cell lysates and tissue lysates.

    This assay employs an antibody specific for rat IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    2x Cell lysate buffer 1 x 5ml
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-rat IL-1 beta 2 vials
    IL-1 beta Microplate (12 x 8 wells) 1 unit
    Recombinant rat IL-1 beta Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Domain
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve of Rt IL1b, with background signal subtracted (duplicates; +/- SD).
  • Rat IL-1b measured in biological fluids or in spleen lysate; background signal subtracted (duplicates +/- SD).
  • Rat IL-1b detected in cell lysates from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma). Results shown for IL-1? levels in 10e6 cells, after background signal was subtracted (duplicates +/- SD).
  • Representative standard curve using ab100768

Protocols

References

This product has been referenced in:
  • Li Z  et al. Calcineurin/nuclear factor-?B signaling mediates isoflurane-induced hippocampal neuroinflammation and subsequent cognitive impairment in aged rats. Mol Med Rep 15:201-209 (2017). ELISA ; Rat . Read more (PubMed: 27909728) »
  • Kang SJ  et al. Inhibitory effects of Persicariae Rhizoma aqueous extracts on experimental periodontitis and alveolar bone loss in Sprague-Dawley rats. Exp Ther Med 12:1563-1571 (2016). Rat . Read more (PubMed: 27588077) »

See all 7 Publications for this product

Customer reviews and Q&As



I can suggest the following general tissue lysate preparation procedure to prepare samples for both the kits.




Tissue lyste samples can be prepared using most conventional methods, for example with RIPA or other formula...

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Yes, that lysis buffer will be good, the 150 mM sodium chloride, 1.0% Triton X-100, 50 mM Tris pH 8.0.


Here are some general guidelines.


Avoid using >0.1% SDS or other strongly denaturing detergents. In general, non-ionic de...

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Da die Tiere ja nur "durchgespült", aber nicht fixiert wurden, sollte dieses Kit mit den Leberlysaten funktionieren.

Vielen Dank für Ihre Anfrage.

Womit perfusieren Sie den die Tiere, PFA oder ein anderes Fixierungsmittel oder einfach nur PBS?

Ich freue mich auf Ihre Antwort.

Thank you for your enquiry. I can confirm that ab100767 IL1 beta Rat ELISA Kit would be the suitable kit to use with plasma samples. ab100768 IL1 beta Rat ELISA Kit is not designed for use with plasma samples. I hope this will be helpful to yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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