Overview

  • Product name
    Rat IL-10 ELISA Kit (Interleukin-10)
    See all IL10 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7%
  • Sample type
    Cell culture supernatant
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 60 pg/ml
  • Range
    0.063 ng/ml - 4 ng/ml
  • Assay time
    4h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-10 (Interleukin-10) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in cell culture supernatants.

    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve Using ab108872.

Protocols

References

ab108872 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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