Overview

  • Product name
    Rat IL-13 ELISA Kit (Interleukin-13)
    See all IL13 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    27.43 pg/ml - 20000 pg/ml
  • Recovery

    114 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 120.4 80% - 139%
    Tissue Extracts 107.8 81% - 135%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-13 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-13 cell lysate and tissue lysate.

    This assay employs an antibody specific for Rat IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease
    Allergic rhinitis
  • Sequence similarities
    Belongs to the IL-4/IL-13 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC30
    • P600
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat IL-13 ELISA Kit (Interleukin-13) images

  • Representative standard curve using ab100766

Protocols

References for Rat IL-13 ELISA Kit (Interleukin-13) (ab100766)

This product has been referenced in:
  • Seiffert J  et al. Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726 (2015). Rat . Read more (PubMed: 25747867) »

See 1 Publication for this product

Product Wall

ab100766 tested now on rat serum

Good Good 4/5 (Ease of Use)
Abreviews
the product worked perfectly on rat serum with the same protocol suggested in the kit for cell culture exctracts, tissue exctracts.
Username

Vasiliki Koumaki

Verified customer

Submitted Jan 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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