|Sample type||Average %||Range|
|Cell culture supernatant||130.2||118% - 138%|
|Plasma||122.8||109% - 138%|
Abcam’s IL-6 (Interleukin-6) Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat IL-6 in plasma or cell culture supernatants.
This assay employs an antibody specific for Rat IL-6 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Due to the relatively low abundance of IL-6 in normal rat plasma, this kit may not be suitable for use with this sample type. We have not been able to detect endogenous Rat IL-6 in normal serum with ab100772, only in serum spiked with Rat IL-6.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Rat IL-6 (lyophilized)||2 vials|
|IL6 Microplate (12 x 8 wells)||1 unit|
|Recombinant Rat IL-6 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|