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Abcam’s IL-6 (Interleukin-6) rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-6 in culture supernatant and serum.
Il-6 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Il-6 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic an stop solution. The density of yellow coloration is directly proportional to the amount of Il-6 captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-rat IL6 monoclonal antibody||1 x 70µl|
|IL-6 Standard lyophilized (4 ng/mL upon reconstitution)||2 vials|
|Microplate coated with monoclonal antibody to rat IL-6 (12 x 8 wells)||1 unit|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119548 has not yet been referenced specifically in any publications.