• Product nameRat IL4 ELISA Set (without plates)
    See all IL4 kits
  • Tests
    5 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    1000 pg/ml - 31.25 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s IL-4 Rat in vitro ELISA Set is designed for the quantitative measurement of Rat IL-4 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


    The rat IL-4 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


    This ELISA Set is intended to measure rat IL-4 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Recognizes both natural and recombinant Rat IL4 Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents


  • RelevanceIL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
  • Cellular localizationSecreted
  • Alternative names
    • B cell stimulatory factor 1
    • Binetrakin
    • BSF1
    • Interleukin 4
  • Database links


Our Abpromise guarantee covers the use of ab48423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notesELISA: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Rat IL4 ELISA Set (without plates) images

    • Rat IL-4 Standard curve ranging from 31.25 to 1000 pg/mL

    References for Rat IL4 ELISA Set (without plates) (ab48423)

    ab48423 has not yet been referenced specifically in any publications.

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