Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay typeSandwich (quantitative)
< 10 pg/ml
156 pg/ml -
Assay durationMultiple steps standard assay
Abcam’s rat Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat Laminin in cell culture supernatants, serum and plasma (heparin, EDTA).
A Laminin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards (form rat sarcoma basement membrane) and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Laminin amount of sample captured in plate.
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Involvement in diseaseDefects in LAMB2 are the cause of Pierson syndrome (PIERSS) [MIM:609049]; also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. Defects in LAMB2 are a cause of congenital nephrotic syndrome (CONPHS) [MIM:609049]. Congenital nephrotic syndrome constitutes a heterogeneous group of conditions having in common the disruption of normal glomerular permselectivity. Congenital nephrotic syndrome due to LAMB2 mutations may be associated with ocular abnormalities.
Sequence similaritiesContains 13 laminin EGF-like domains. Contains 1 laminin IV type B domain. Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. Domains VI and IV are globular.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.