• Product name
    Rat Leptin ELISA Kit
    See all Leptin kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    10.97 pg/ml - 8000 pg/ml
  • Recovery

    84 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.43 68% - 89%
    Serum 92.32 83% - 102%
    Plasma 84.25 75% - 94%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Leptin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Function
    May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
  • Involvement in disease
    Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • Sequence similarities
    Belongs to the leptin family.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • FLJ94114
    • LEP
    • LEPD
    • Leptin
    • Leptin (murine obesity homolog)
    • Leptin (obesity homolog, mouse)
    • Leptin Murine Obesity Homolog
    • Leptin Precursor Obesity Factor
    • OB
    • Obese protein
    • Obese, mouse, homolog of
    • Obesity
    • Obesity factor
    • Obesity homolog mouse
    • Obesity Murine Homolog Leptin
    • OBS
    • OTTHUMP00000212285
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat Leptin ELISA Kit images

  • Rat Leptin measured in supernatanses showing quantity (ng) per mL of tested sample

  • Rat Leptin measured in biofluids showing quantity (ng) per mL of tested sample

  • Standard curve: mean of duplicates (+/-SD) with background readings subtracted


References for Rat Leptin ELISA Kit (ab100773)

This product has been referenced in:
  • Park JH  et al. Blackcurrant Suppresses Metabolic Syndrome Induced by High-Fructose Diet in Rats. Evid Based Complement Alternat Med 2015:385976 (2015). Sandwich ELISA ; Rat . Read more (PubMed: 26504474) »

See 1 Publication for this product

Product Wall

Abreview for Leptin Rat ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Rat Serum (Blood)
Specification: Blood
Amount of Sample: 50 µg
Type of Kit Used: ELISA
Positive control: rat serum (diabetic)
Did you use the Abcam Kit protocol?: Yes

Dr. Asmat Salim

Verified customer

Submitted May 07 2012

Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laborat...

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