|Sample type||Average %||Range|
|Cell culture supernatant||75.43||68% - 89%|
|Serum||92.32||83% - 102%|
|Plasma||84.25||75% - 94%|
Abcam’s Leptin Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|5X Assay Diluent D||1 x 15ml|
|Biotinylated anti-rat Leptin||2 vials|
|Leptin Microplate (12 x 8 wells)||1 unit|
|Recombinant rat Leptin Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Rat Leptin measured in supernatanses showing quantity (ng) per mL of tested sample
Rat Leptin measured in biofluids showing quantity (ng) per mL of tested sample
Standard curve: mean of duplicates (+/-SD) with background readings subtracted