Rat Lipocalin-2 ELISA Kit (NGAL) (ab119602)

Overview

  • Product name
    Rat Lipocalin-2 ELISA Kit (NGAL)
    See all Lipocalin-2 / NGAL kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human, Pig
  • Product overview

    Abcam’s rat Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat Lipocalin-2 in cell culture supernatants, serum plasma (heparin) and urine.

    A Lipocalin-2 specific mouse monoclonal antibody for Lipocalin-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Lipocalin-2 amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Colorimetric standard curve: mean of duplicates (+/-) with background readings subtracted

  • NGAL (ng per mg protein) measured in tissue lysates

  • Lipocalin-2 measured in biological fluids showing quantity (ng) per mL of tested sample

Protocols

References

ab119602 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ELISA
Sample
Rat Tissue sections (Cortical lysate)
Type of Kit Used
ELISA
Specification
Cortical lysate
Did you use the Abcam Kit protocol?
Yes
Amount of Sample
10 µg
Username

Abcam user community

Verified customer

Submitted Jan 07 2016

Thank you for contacting us and for your patience in this matter.

I had the lab look at the data and they think concentration of the samples are too high, that caused the hook effect.
Thus we suggest to perform serial dilutions for serum ...

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Thank you for contacting us.

Because the working concentration and the sensitivity is different in WB and ELISA, we don't suggest to use the antibodies in our Elisa kit in WB.

I hope this information is helpful to you. Please do not h...

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I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Li...

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Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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Thank you for contacting us. It is my pleasure to help.

We do offer a KIM1 rat ELISA kit as well. This kit has a similar system containing one plate made of twelve 8 well strips. The product number is ab119597 and may be found at the follo...

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Thank you for contacting us.

ab119602 Lipocalin-2 / NGAL Rat ELISA Kitcontains one 96-well plate precoated with anti- rat NGAL antibody with dismountable and detachable twelve 8-well strips.

I hope this information is helpful to...

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Yes, this NGAL Elisa kit can test urine.



I hope this is helpful. Please contact me again if you have any further questions.

Thank you for contacting us.

First, I want to apologize to you for the delay in gathering this information. I hope this did not cause you any inconveniences.

In regards to using rat tissues in our SCF Mouse ELISA kit, there is no ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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