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Abcam’s rat Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat Lipocalin-2 in cell culture supernatants, serum plasma (heparin) and urine.
A Lipocalin-2 specific mouse monoclonal antibody for Lipocalin-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Lipocalin-2 amount of sample captured in plate.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-rat Lipocalin-2 antibody Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti-rat Lipocalin-2 antibody||1 x 130µl|
|Lyophilized recombinant rat Lipocalin-2 standard||2 x 10ng|
|Plate Seal||1 x 4 units|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab119602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Colorimetric standard curve: mean of duplicates (+/-) with background readings subtracted
NGAL (ng per mg protein) measured in tissue lysates
Lipocalin-2 measured in biological fluids showing quantity (ng) per mL of tested sample
ab119602 has not yet been referenced specifically in any publications.