Rat Liver Mitochondrial lysate (ab110346)

Overview

  • Product nameRat Liver Mitochondrial lysate
    See all Rat Liver lysates
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.


    2 mg of purified rat liver mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • Tested applicationsSuitable for: SDS-PAGE, WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferConstituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • Lysate notes2 mg of purified rat liver mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

    Applications

    Our Abpromise guarantee covers the use of ab110346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    SDS-PAGE Use at an assay dependent dilution.
    WB Use at an assay dependent dilution.

    References for Rat Liver Mitochondrial lysate (ab110346)

    ab110346 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. Please find below the further requested information.

    The composition is 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose, pH 7.8

    The concentration of mitochondrial lysate is 2mg at 5mg/ml.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"