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Product was previously marketed under the MitoSciences sub-brand.
2 mg of purified rat liver mitochondrial membranes resuspended in 400 µl of heart mitochondria resuspension buffer (10 mM Tris.HCl, pH 7.8, 0.2 M sucrose, 0.2 mM EDTA, 1 mM PMSF). 1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.
Our Abpromise guarantee covers the use of ab110346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|SDS-PAGE||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
ab110346 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"