Rat Metabotropic Glutamate Receptor 5 peptide (ab17575)



  • Nature


Our Abpromise guarantee covers the use of ab17575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Glutamate receptor metabotropic 5
    • GPRC1E
    • Grm5
    • GRM5_HUMAN
    • Metabotropic glutamate receptor 5
    • Metabotropic glutamate receptor 5 variant F
    • Metabotropic glutamate receptor 5 variant G
    • Metabotropic glutamate receptor 5 variant H
    • mGlu5
    • mGluR5
    • PPP1R86
    • Protein phosphatase 1 regulatory subunit 86
    see all
  • Function
    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

Rat Metabotropic Glutamate Receptor 5 peptide images

  • Performed under reducing conditions.

    This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    Both the positive control antibody, Rabbit polyclonal to mGluR5 in lane 1 and ab16653, Rabbit polyclonal to mGluR5 (phospho S996) in lanes 2 and 4, detect an ~130kDa band in mouse hippocampal lysate by western blot. We are unsure of the identity of the larger >200kDa band that is seen with both the postive control antibody and also the ab16653 phospho specific antibody (lanes 1, 2 and 4).

    The ~130kDa and >200kDa bands detected by ab16653 mGluR5 (phospho S996) antibody were completely blocked by preincubation with the mGluR5 (phsopho S996) immunizing peptide (ab17573; lane 3). However as expected, the corresponding unmodified control peptide (ab17575) did not block ab16653 detection of mGluR5 (phospho S996) protein expression.

References for Rat Metabotropic Glutamate Receptor 5 peptide (ab17575)

ab17575 has not yet been referenced specifically in any publications.

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