Anti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (FITC) (ab111922)

Overview

  • Product name
    Anti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (FITC)
    See all RAT MHC CLASS II RT1D primary antibodies
  • Description
    Mouse monoclonal [MRC OX-17] to RAT MHC CLASS II RT1D (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat spleen membrane glycoproteins depleted of Ia-A antigens.

  • Positive control
    • Rat spleen cells (Fischer strain).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab111922 was purified from ascitic fluid via Protein G Chromatography
  • Clonality
    Monoclonal
  • Clone number
    MRC OX-17
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.05-0.1µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Rat RT1D is MHC CLASS II, the rat homologue of mouse I-E, and is present on all rat strains.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • H2-Ea antibody
    • Histocompatibility 2, class II antigen E alpha antibody
    • MGC112637 antibody
    • MHC class II antigen RT1.D alpha chain antibody
    • MHC class II RT1D alpha chain antigen antibody
    • MHC RT1.u D alpha chain antibody
    • RT1 class II, locus Da antibody
    • RT1 D alpha antibody
    • RT1 Du alpha antibody
    • RT1-u antibody
    see all

Anti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (FITC) images

  • ab111922 at 0.1 µg/106 cells detecting RAT MHC CLASS II RT1D in Fischer Rat spleen cells by Flow Cytometry. 49% more of the cells were stained than in the control (FITC Mouse IgG1).

References for Anti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (FITC) (ab111922)

This product has been referenced in:
  • Fukumoto T  et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 (1982). Read more (PubMed: 6178598) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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