Anti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (Phycoerythrin) (ab111825)

Overview

  • Product nameAnti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (Phycoerythrin)
    See all RAT MHC CLASS II RT1D primary antibodies
  • Description
    Mouse monoclonal [MRC OX-17] to RAT MHC CLASS II RT1D (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab111825 recognizes a monomorphic determinant on the a chain of the Rat Ia antigen and appears to be the rat homologue of Mouse Ia-E. It recognizes the Rat Ia product present on B, but not T cells from lymph node or thoracic duct lymph. It does not bind to thymocytes or erythrocytes. ab111825 does not cross-react with Rat Ia-A or Mouse Ia-E antigen, but rabbit antibody raised against the antibody affinity column-purified MRC OX-17 antigen cross-reacted on tissues of mice expressing Ia-E mouse antigen but not on those mouse strains that were Ia-E antigen negative.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat spleen membrane glycoproteins depleted of Ia-A antigens.

  • Positive control
    • Rat stain buffalo spleen cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab111825 is purified from ascitic fluid via Protein G Chromatography.
  • ClonalityMonoclonal
  • Clone numberMRC OX-17
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceRat RT1D is MHC CLASS II, the rat homologue of mouse I-E, and is present on all rat strains.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • H2-Ea antibody
    • Histocompatibility 2, class II antigen E alpha antibody
    • MGC112637 antibody
    • MHC class II antigen RT1.D alpha chain antibody
    • MHC class II RT1D alpha chain antigen antibody
    • MHC RT1.u D alpha chain antibody
    • RT1 class II, locus Da antibody
    • RT1 D alpha antibody
    • RT1 Du alpha antibody
    • RT1-u antibody
    see all

Anti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (Phycoerythrin) images

  • ab111825 at 0.5 µg/106 cells staining Rat MHC CLASS II RT1D in Rat stain Buffalo spleen cells by Flow Cytometry. 49.6% more cells were stained that in the negative control.

References for Anti-RAT MHC CLASS II RT1D antibody [MRC OX-17] (Phycoerythrin) (ab111825)

ab111825 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"