Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesRat
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab39304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Zymography

    Western blot

    SDS-PAGE

  • FormLiquid
  • Additional Notes


    This protein degrades denatured collagen (gelatin) and a range of extracellular matrix components in vivo. The protein was tested for activity in a zymogram (1 mg/mL gelatin in a 10% acrylamide gel). The smallest amount loaded was 1 ng.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, PBS, 75mM Sodium chloride, pH 7.4

General info

  • Alternative names
    • 72 kDa gelatinase
    • 72kD type IV collagenase
    • CLG 4
    • CLG 4A
    • CLG 4A
    • CLG4
    • CLG4A
    • CLG4A
    • Collagenase Type 4 alpha
    • Collagenase Type 4 alpha
    • Collagenase type IV A
    • Gelatinase A
    • Gelatinase alpha
    • Gelatinase alpha
    • Gelatinase neutrophil
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase
    • Matrix Metalloproteinase 2
    • Matrix Metalloproteinase 2
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
    • Matrix metalloproteinase II
    • Matrix metalloproteinase-2
    • MMP 2
    • MMP 2
    • MMP II
    • MMP-2
    • MMP2
    • MMP2_HUMAN
    • MONA
    • Neutrophil gelatinase
    • PEX
    • TBE 1
    • TBE-1
    see all
  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt

References for Rat MMP2 full length protein (ab39304)

This product has been referenced in:
  • Hawkins KE  et al. Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke. Mol Brain 6:14 (2013). Read more (PubMed: 23522154) »
  • Rosewell K  et al. Identification of hepsin and protein disulfide isomerase A3 as targets of gelatinolytic action in rat ovarian granulosa cells during the periovulatory period. Biol Reprod 85:858-66 (2011). Read more (PubMed: 21734266) »

See all 2 Publications for this product

Product Wall

Application Western blot
PAGE: 7.5%, reducing-denaturing
up to 1 ug total protein loaded
Blocking with 10% milk overnight
Primary antibody: ab37150 dilution 1:1000
Secondary antibody: goat biotinylated anti rabbit dilution 1200
Detection: ABC system
No Bands
Contacted abcam on 10-19-12 and they offered a free replacement with ab39302 that worked.
Username

Mrs. Henda Nabli

Verified customer

Submitted Nov 05 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for taking time to contact us. I am sorry to hear this protein is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality....

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Thank you for contacting Abcam.
You should not have any issues using ab39304 with ab37150 as a positive control in western blot. Both are covered under our Abpromise for six months and are guaranteed to work in western blot.
If there is anythi...

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Thanks for your interst in ab39304.

At this time we know that this rat MMP2 protein is active and has been used in zymography. On the datasheet this is described as:

"Zymography: Use at an assay dependent concentration. The Rat...

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Thank you for your call today and for letting us know about the trouble with this protein.

I had requested that a replacement vial be sent to you, but your colleague kindly called to let us know that we have previously credited your account f...

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Thank you for your reply and understanding.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

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Thank you for your enquiry and for your patience. I am sorry to hear you have some concerns regarding this product.

I can confirm that this particular product is sourced externally. I have been in contact with them in order to obtain this in...

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Thank you for your message and for your patience.

I sincerely apologise for the delay. I have contacted our source to request the information regarding the purification of the product, but they have regrettably not yet got back to me. I will...

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Thank you for contacting us.

We do have several MMP proteins in our catalog.

We have for MMP2:

ab39302 (human):Full length Human MMP2 protein (purified from human fibroblast cell culture media). It has not yet been tested...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"