Overview

  • Product name
    Rat MMP8 ELISA Kit
    See all MMP8 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 50 pg/ml
  • Range
    0.05 ng/ml - 40 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.42 83% - 108%
    Serum 109.78 99% - 118%
    Plasma 107.6 98% - 118%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s MMP8 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP8 in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.

    This assay employs an antibody specific for MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100779

  • Representative standard curve using ab100779

Protocols

References

This product has been referenced in:
  • Monaghan M  et al. A collagen-based scaffold delivering exogenous microrna-29B to modulate extracellular matrix remodeling. Mol Ther 22:786-96 (2014). Read more (PubMed: 24402185) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your patience.

The lab let me know the following:

ab100609 : Cross reactivity with mouse is unknown. If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal.

ab100779 : Untes...

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Thank you for contacting us.

I have contacted the lab regarding the cross-reactivity of the 2 kits and will let you know what I find out.

As for the testing discount program, we have the following general information:

For...

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Thank you for your enquiry.

ab100779 does not measure MMP8 activity. It is a sandwich ELISA assay that just quantitates amount of protein present.

I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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