Rat monoclonal [11-26] Anti-Mouse IgD delta chain (FITC) (ab99566)

Overview

  • Product name
    Rat monoclonal [11-26] Anti-Mouse IgD delta chain (FITC)
    See all IgD secondary antibodies
  • Host species
    Rat
  • Target species
    Mouse
  • Specificity
    ab99566 reacts with mouse IgD of all haplotypes (e.g., IgDa, IgDb, IgDc), and does not react with other immunoglobulin isotypes. ab99566 does not induce proliferation of mature B cells in vitro, nor does in vivo injection of the monoclonal antibody have any effect on activation of mature B lymphocytes.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

Applications

Our Abpromise guarantee covers the use of ab99566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Images

  • BALB/c splenocytes were double-stained with ab99566 and Rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed using a FACScanTM flow cytometer.

References

This product has been referenced in:
  • Hamilton AM  et al. Immunofluorescence analysis of B-1 cell ontogeny in the mouse. Int Immunol 6:355-61 (1994). Read more (PubMed: 7514440) »
  • Nitschke L  et al. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. Proc Natl Acad Sci U S A 90:1887-91 (1993). Read more (PubMed: 8446604) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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