Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Alkaline Phosphatase) (ab99631)

Overview

  • Product nameRat monoclonal H139-52.1 Anti-Mouse kappa light chain (Alkaline Phosphatase)
    See all Kappa light chain secondary antibodies
  • Target speciesMouse
  • Specificityab99631 reacts with mouse kappa light chains
  • Tested applicationsSuitable for: ELISA, Flow Cyt, ICC/IF, WBmore details
  • ConjugationAlkaline Phosphatase

Properties

Applications

Our Abpromise guarantee covers the use of ab99631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/1000.
Flow Cyt Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Alkaline Phosphatase) images

  • Comparison of an anti-mouse secondary antibody recognizing the H&L chain versus (ab97020) versus a mouse light-chain specific secondary antibody (ab99631)


    Left blot: AbExcel Goat polyclonal Secondary Antibody to Mouse IgG – H&L (AP) (ab97020), 1/50,000

    Lane 1: 0.5 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 2: 0.25 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 3: 0.125 µg mouse monoclonal to SOX10 (ab108365) was loaded

    Right blot: Rat monoclonal Secondary Antibody to Mouse kappa - light chain (AP) (ab99631),1/10,000

    Lane 1: 2 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 2: 1 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 3: 0.1 µg mouse monoclonal to SOX10 (ab108365) was loaded

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (AP) (ab99631) at 1/5000 dilution
    Lanes 3 - 4 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (AP) (ab99631) at 1/20000 dilution
    Lanes 5 - 6 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (AP) (ab99631) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 4 minutes

References for Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Alkaline Phosphatase) (ab99631)

ab99631 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99631.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"