Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Alkaline Phosphatase) (ab99631)

Overview

  • Product name
    Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Alkaline Phosphatase)
    See all Kappa light chain secondary antibodies
  • Target species
    Mouse
  • Specificity
    ab99631 reacts with mouse kappa light chains
  • Tested applications
    Suitable for: ELISA, Flow Cyt, ICC/IF, WBmore details
  • Conjugation
    Alkaline Phosphatase

Properties

Applications

Our Abpromise guarantee covers the use of ab99631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/1000.
Flow Cyt Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent concentration.

Images

  • Comparison of an anti-mouse secondary antibody recognizing the H&L chain versus (ab97020) versus a mouse light-chain specific secondary antibody (ab99631)


    Left blot: AbExcel Goat polyclonal Secondary Antibody to Mouse IgG – H&L (AP) (ab97020), 1/50,000

    Lane 1: 0.5 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 2: 0.25 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 3: 0.125 µg mouse monoclonal to SOX10 (ab108365) was loaded

    Right blot: Rat monoclonal Secondary Antibody to Mouse kappa - light chain (AP) (ab99631),1/10,000

    Lane 1: 2 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 2: 1 µg mouse monoclonal to SOX10 (ab108365) was loaded
    Lane 3: 0.1 µg mouse monoclonal to SOX10 (ab108365) was loaded

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (AP) (ab99631) at 1/5000 dilution
    Lanes 3 - 4 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (AP) (ab99631) at 1/20000 dilution
    Lanes 5 - 6 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (AP) (ab99631) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 4 minutes

References

ab99631 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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