Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (HRP) (ab99632)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab99632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ELISA 1/1000 - 1/2000.
WB Use at an assay dependent concentration.

Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (HRP) images

  • Comparison of an anti-mouse secondary antibody recognizing the H&L chain versus (ab97023) versus a mouse light chain specific secondary antibody (ab99632)


    Left blot: AbExcel Goat polyclonal Secondary Antibody to Mouse IgG – H&L (HRP) (ab97023), 1/50,000

    Lane 1: 0.5 µg mouse monoclonal was loaded
    Lane 2: 0.25 µg mouse monoclonal was loaded
    Lane 3: 0.125 µg mouse monoclonal was loaded

    Right blot: Rat monoclonal Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632),1/10,000

    Lane 1: 2 µg mouse monoclonal was loaded
    Lane 2: 1 µg mouse monoclonal was loaded
    Lane 3: 0.1 µg mouse monoclonal was loaded

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632) at 1/2000 dilution
    Lanes 3 - 4 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632) at 1/10000 dilution
    Lanes 5 - 6 : Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa - light chain (HRP) (ab99632) at 1/20000 dilution

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

    Exposure time : 20 seconds
  • Lane 1: Mouse IgG1 Kappa loaded at 0.2ug/well
    Lane 2: Mouse IgG3 Lambda loaded at 0.2ug/well
    ab99632 diluted to 1:2,000

References for Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (HRP) (ab99632)

ab99632 has not yet been referenced specifically in any publications.

Product Wall


As we discussed, for purified primary antibodies such as ab70475 we recommend a starting dilution of 1ug/ml for western blot. For the secondary antibody ab99632, we were able to see a band in western blot from a dilution range of 1:2,000 to 1:10,0...

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Thank you very much for your inquiry.
Indeed the ab99632 will solve your problem and not recognize any heavy chain! I can therefore confirm that this is the best choice in my opinion.
There would be alternative solutions, however I think the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"