Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Phycoerythrin) (ab99635)

Overview

  • Product name
    Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Phycoerythrin)
    See all Kappa light chain secondary antibodies
  • Description
    Rat monoclonal [H139-52.1] Secondary Antibody to Mouse kappa light chain (PE)
  • Target species
    Mouse
  • Specificity
    ab99635 reacts with kappa light chains.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, ELISAmore details
  • Immunogen

    Other Immunogen Type corresponding to Kappa light chain.

  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

Applications

Our Abpromise guarantee covers the use of ab99635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Images

  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Phycoerythrin) ab99635 (0.1 µg/106cells) and Rat Anti-Mouse CD19-FITC.
  • Flow Cytometry analysis of BALB/c mouse splenocytes stained with Rat monoclonal H139-52.1 Anti-Mouse kappa light chain (Phycoerythrin) ab99635 (0.1 µg/106 cells) and Rat Anti-Mouse CD19-FITC.

  • Cells from BALB/c spleen were double stained with ab99635 (0.1 µg/106 cells) and Rat anti-Mouse CD3e-FITC following which small lymphocytes were gated and analyzed using a FACScanTM flow cytometer (BDIS, San Jose, CA).

References

ab99635 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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