Rat monoclonal LO-MG2b-2 Anti-Mouse IgG2b heavy chain (Biotin) (ab99640)

Overview

  • Product name
    Rat monoclonal LO-MG2b-2 Anti-Mouse IgG2b heavy chain (Biotin)
    See all IgG2b secondary antibodies
  • Description
    Rat monoclonal [LO-MG2b-2] Secondary Antibody to Mouse IgG2b - heavy chain (Biotin)
  • Target species
    Mouse
  • Specificity
    ab99640 reacts with the γ2b heavy chain of mouse IgG2b. Does not react with other immunoglobulin isotypes.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, ELISAmore details
  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    LO-MG2b-2
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18403-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Rat monoclonal LO-MG2b-2 Anti-Mouse IgG2b heavy chain (Biotin) images

  • Top: (Negative control) ab99640 being used to stain Human blood at 1 ug/106 with Streptavidin-FITC
    Bottom: ab99640 staining Human blood incubated with mouse anti-human CD45RA (IgG2b isotype) at 1 ug/106 with Streptavidin-FITC.

References for Rat monoclonal LO-MG2b-2 Anti-Mouse IgG2b heavy chain (Biotin) (ab99640)

ab99640 has not yet been referenced specifically in any publications.

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Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recogn...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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