Rat monoclonal SB77e Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed (ab99602)

Overview

  • Product name
    Rat monoclonal SB77e Anti-Mouse IgG1 H&L (Alkaline Phosphatase) preadsorbed
    See all IgG1 secondary antibodies
  • Description
    Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (AP), pre-adsorbed
  • Target species
    Mouse
  • Specificity
    ab99602 reacts with Mouse IgG1 as demonstrated by ELISA.
  • Tested applications
    Suitable for: ELISA, Flow Cytmore details
  • Minimal
    cross-reactivity

    Goat, Human, Rabbit more details
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 50mM Tris, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    SB77e
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/1000.
Flow Cyt Use at an assay dependent dilution.

References

This product has been referenced in:
  • Sircar G  et al. Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES. J Biol Chem 291:18016-29 (2016). Read more (PubMed: 27358405) »

See 1 Publication for this product

Customer reviews and Q&As

The calf intestinal alkaline phosphatase conjugated to ab127427 is purified from healthy bovine material. It is not a recombinant protein.

I hope this is helpful. Let us know if you have any questions or if we may assist you further.

Thanks for your reply, although I'm afraid I still don't understand what information you are looking for. Are you looking for the sequence of the gene that expresses the alkaline phosphatase that is conjugated to ab99602?

Thank you for contacting Abcam.

I am not familiar with the assay that you are using and so I am not sure if I can answer your first question, but I will speak to some of my colleagues and see if there is anything they can suggest. I will let ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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