Rat monoclonal SB77e Anti-Mouse IgG1 H&L (Phycoerythrin) preadsorbed (ab99605)

Overview

  • Product nameRat monoclonal SB77e Anti-Mouse IgG1 H&L (Phycoerythrin) preadsorbed
    See all IgG1 secondary antibodies
  • Description
    Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (PE), pre-adsorbed
  • Target speciesMouse
  • Specificityab99605 reacts with Mouse IgG1 as demonstrated by ELISA. Each batch is tested to show minimal cross reactivity with IgM, IgA, and other subsets of IgG.
  • Tested applicationsSuitable for: ELISA, Flow Cyt, ICC/IFmore details
  • Minimal
    cross-reactivity

    Goat, Human, Rabbit more details
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

Applications

Our Abpromise guarantee covers the use of ab99605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ICC/IF Use 0.1µg for 106 cells.

Rat monoclonal SB77e Anti-Mouse IgG1 H&L (Phycoerythrin) preadsorbed images

  • Top: (Negative control) ab99605 being used against Mouse IgG1 in human blood at 0.1 µg/106 cells.
    Bottom: ab99605 staining human blood incubated with mouse anti-human CD3 (IgG1 isotype) at 0.1 µg/106 cells.

References for Rat monoclonal SB77e Anti-Mouse IgG1 H&L (Phycoerythrin) preadsorbed (ab99605)

ab99605 has not yet been referenced specifically in any publications.

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Antibodies are sometimes pre-adsorbed. This means they have been adsorbed with other proteins, or serum from various species, etc, to eliminate any antibody that may cross-react. The resulting purified antibody s...

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As shown on the datasheet, the primary antibody ab15 is a Mouse IgG1. Therefore I confirm that ab99605 is compatible with ab15.

I hope this information is helpful to you. Please do not hesitate to contact ...

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Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - ra...

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Thank you for contacting us. The antibodies I recommend are: PPAR alpha rabbit polyclonal - ab61182 Secondary - goat anti-rabbit IgG Dylight 488-conjugated, pre-adsorbed ab96899 CD4 mouse monoclonal - ab25804 Secondary - rat anti-mouse IgG1 PE-co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"