Rat monoclonal SB84a Anti-Mouse IgG2a gamma chain (FITC) (ab79092)

Overview

  • Product nameRat monoclonal SB84a Anti-Mouse IgG2a gamma chain (FITC)
    See all IgG2a secondary antibodies
  • Target speciesMouse
  • SpecificityReacts with the heavy chain of IgG2a
  • Tested applicationsSuitable for: ELISA, Flow Cyt, ICC/IFmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive controlHuman PBMC.
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

Applications

Our Abpromise guarantee covers the use of ab79092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.



ab18404-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Rat monoclonal SB84a Anti-Mouse IgG2a gamma chain (FITC) images

  • Top: (Negative control) ab79092 being used against human blood at 1 µg/106 cells.
    Bottom: ab99605 staining human blood incubated with mouse anti-human CD5 (IgG2a isotype) at 1 µg/106 cells.
  • Human PBMC were incubated with either mouse IgG1 anti-CD3, mouse IgG2a anti-CD5, mouse IgG2b anti-CD22 or mouse IgG3 anti-IgD. After washing the cells were then incubated with ab79092 at 1µg/106 cells. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

References for Rat monoclonal SB84a Anti-Mouse IgG2a gamma chain (FITC) (ab79092)

This product has been referenced in:
  • Mimura K  et al. Therapeutic potential of highly cytotoxic natural killer cells for gastric cancer. Int J Cancer 135:1390-8 (2014). Read more (PubMed: 24615495) »

See 1 Publication for this product

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The best anti mouseIgG2a secondaries we can offer are ab11582, ab97244, ab79092and ab98697.

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