Overview

Description

  • NatureSynthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab32297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityDue to sequence homology, antibody (ab32298)generated against immunising peptide ab32297 will detect Nogo isoforms A and B (Foocen M1), and is predicted to react with the additional isoforms Nogo C and Foocen M2.
  • Applications

    Blocking - Blocking peptide for Anti-Nogo antibody (ab32298)

  • FormLiquid
  • Additional notesDue to sequence homology, antibody (ab32298)generated against immunising peptide ab32297 will detect Nogo isoforms A and B (Foocen M1), and is predicted to react with the additional isoforms Nogo C and Foocen M2.

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • 1110020G17Rik
    • AA407876
    • AA409940
    • AA960376
    • ASY
    • C130026I10Rik
    • Foocen
    • Glut4 vesicle 20 kDa protein
    • Human NogoA
    • Kiaa0886
    • KIAA4153
    • MGC116054
    • MGC139261
    • mKIAA0886
    • mKIAA4153
    • My043 protein
    • Nbla00271
    • Nbla10545
    • Neurite growth inhibitor 220
    • Neurite Growth Inhibitor 220, included
    • Neurite outgrowth inhibitor
    • Neuroendocrine-specific protein
    • Neuroendocrine-specific protein C homolog
    • NI-250
    • NI220/250
    • NOGO
    • Nogo A
    • Nogo B
    • Nogo C
    • Nogo protein
    • NOGOC
    • NSP
    • NSP-CL
    • rat N
    • Reticulon 4
    • Reticulon 5
    • Reticulon-4
    • Reticulon-5
    • RTN X
    • RTN-x
    • Rtn4
    • Rtn4 reticulon 4
    • RTN4-A
    • RTN4-B1
    • RTN4-B2
    • RTN4-C
    • RTN4_HUMAN
    • Vp20
    see all
  • FunctionDevelopmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
  • Tissue specificityIsoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
  • Sequence similaritiesContains 1 reticulon domain.
  • DomainThree regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
  • Cellular localizationEndoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
  • Information by UniProt

References for Rat Nogo peptide (ab32297)

ab32297 has not yet been referenced specifically in any publications.

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