The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityDue to sequence homology, antibody (ab32298)generated against immunising peptide ab32297 will detect Nogo isoforms A and B (Foocen M1), and is predicted to react with the additional isoforms Nogo C and Foocen M2.
Blocking - Blocking peptide for Anti-Nogo antibody (ab32298)
Additional notesDue to sequence homology, antibody (ab32298)generated against immunising peptide ab32297 will detect Nogo isoforms A and B (Foocen M1), and is predicted to react with the additional isoforms Nogo C and Foocen M2.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Glut4 vesicle 20 kDa protein
Neurite growth inhibitor 220
Neurite Growth Inhibitor 220, included
Neurite outgrowth inhibitor
Neuroendocrine-specific protein C homolog
Rtn4 reticulon 4
FunctionDevelopmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
Tissue specificityIsoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Sequence similaritiesContains 1 reticulon domain.
DomainThree regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
Cellular localizationEndoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.