Overview

  • Product name
    Rat PDGF-AA ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type
    Serum, Plasma, Cell culture media
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 90 pg/ml
  • Range
    93.75 pg/ml - 6000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 100 91% - 108%
    Plasma 101.6 84% - 114%
    Cell culture media 130.4 122% - 138%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s PDGF-AA Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of rat PDGF-AA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for rat PDGF-AA coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat PDGF-AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    280X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-rat PDGF-AA 2 vials
    PDGF-AA Microplate (12 x 8 wells) 1 unit
    Recombinant Rat PDGF-AA Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Subunit structure: Homodimer (AA); antiparallel disulfide-linked dimer. Heterodimer with PDGFB (AB); antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4.
  • Alternative names
    • PDGF A
    • PDGF A chain
    • PDGF subunit A
    • PDGF1
    • platelet derived growth factor A chain
    • platelet derived growth factor alpha chain
    • platelet derived growth factor alpha isoform 2 preproprotein
    • platelet derived growth factor subunit A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab155464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155464 -  Assay Diluent A.

  • Representative standard curve using ab155464 - Assay Diluent B.

Protocols

References

ab155464 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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