Overview

  • Product nameRat Prolactin R ELISA Kit (PRLR)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.4 ng/ml
  • Range
    0.512 ng/ml - 50 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 125.4 117% - 141%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Prolactin R Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Prolactin R in cell culture supernatants.

    This assay employs an antibody specific for rat Prolactin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Prolactin R antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Note: very low signal were obtained in normal serum and plasma

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionThis is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • Tissue specificityExpressed in breast, placenta, kidney, liver and pancreas.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationSecreted and Membrane.
  • Information by UniProt
  • Alternative names
    • PRL-R
    • PRLR
    • PRLR_HUMAN
    • Prolactin receptor
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat Prolactin R ELISA Kit (PRLR) images

  • Representative standard curve using ab113351

Protocols

References for Rat Prolactin R ELISA Kit (PRLR) (ab113351)

This product has been referenced in:
  • Adedara IA  et al. Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats. Arch Environ Contam Toxicol N/A:N/A (2014). Read more (PubMed: 25179371) »

See 1 Publication for this product

Product Wall

Thank you for your interest in our Prolactin R Rat ELISA, ab113351. The laboratory that developed the ELISA has not tested it on milk or pituitary samples, but does not expect that there will be any problems. We recommend following the kit protocol sug...

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The laboratory has confirmed that this kit will not detect mouse Prolactin R. The rabbit polyclonal capture antibody will, but not the mouse monoclonal detection antibody.

Gracias por contactarnos.


El anticuerpo usado como captura cubriendo la placa procede de cabra, y es policlonal. El otro anticuerpo, el de detección se ha producido en ratón, y es monoclonal.

Espero haberte ayudad...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"