|Sample type||Average %||Range|
|Plasma||74.84||69% - 88%|
|Cell culture media||64.74||53% - 70%|
Abcam’s RAGE Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat RAGE in plasma and cell culture supernatants.
This assay employs an antibody specific for Rat RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Rat RAGE in normal serum with ab100780, only in serum spiked with Rat RAGE.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|5X Assay Diluent D||1 x 15ml|
|Biotinylated anti-rat RAGE||2 vials|
|RAGE Microplate (12 x 8 wells)||1 unit|
|Recombinant rat RAGE Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100780 has not yet been referenced specifically in any publications.