Overview

  • Product name
    Rat RAGE ELISA Kit
    See all RAGE kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 300 pg/ml
  • Range
    0.41 ng/ml - 300 ng/ml
  • Recovery

    72 %

    Sample specific recovery
    Sample type Average % Range
    Plasma 74.84 69% - 88%
    Cell culture media 64.74 53% - 70%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s RAGE Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat RAGE in plasma and cell culture supernatants.


    This assay employs an antibody specific for Rat RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Rat RAGE in normal serum with ab100780, only in serum spiked with Rat RAGE.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100780

Protocols

References

This product has been referenced in:
  • Hurtgen BJ  et al. Autologous minced muscle grafts improve endogenous fracture healing and muscle strength after musculoskeletal trauma. Physiol Rep 5:N/A (2017). Functional Studies ; Rat . Read more (PubMed: 28747511) »

See 1 Publication for this product

Customer reviews and Q&As

The laboratory has informed me that ab100780 will detect soluble RAGE.

Thank you for contacting us.


Although this particular kit was neither designed nor validated with lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test th...

Read More

Thank you for calling Abcam yesterday.

I have talked to the lab and we should be able to add the standard from ab100780 to the catalogue. It may take a week or so though. I will contact you with the abID when it is ready to be ordered.
<...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up