|Sample type||Average %||Range|
|Cell culture supernatant||105||95% - 118%|
|Serum||100||95% - 106%|
Abcam’s TGF beta 1 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Rat TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA and heparin).
Rat TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Rat TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rat TGF beta 1 captured on the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||2 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||6 units|
|Biotin-Conjugate anti-rat TGF beta 1 monoclonal antibody||1 x 120µl|
|Microplate coated with monoclonal antibody to rat TGF beta 1 (12 x 8 wells)||1 unit|
|Rat TGF beta 1 Standard Lyophilized||2 vials|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Activated TGF-beta 1 measured in tissue lysates, homogenised in RIPA buffer, activated (20 uL of HCl per 20 uL of samples with 180 uL of buffer) and tested between 25-250 ug protein per mL. Data expressed as TGF beta per mg of extracted protein (duplicates +/- SD).
Activated TGF-beta 1 measured in rat biological fluids (dilution range 1/50-1/200; duplicates +/- SD). Human and mouse samples, diluted 1:12, gave results below the level of detection.
Sandwich ELISA on rat blood serum using Abcam's TGF beta 1 Rat ELISA Kit (ab119558).
Representative Standard Curve using ab119558.